Shimadzu UV-VIS SPD-20A/20AV LC Detectors  

Offering dual-wavelength mode
The SPD-20A/20AV is a UV-VIS detector that takes sensitivity to the limit (Noise level 0.5 x 10(-5) AU), and offers wide linearity (2.5AU). SPD-20A: Wavelength range: 190 to 700nm The SPD-20AV has a mode that allows the deuterium lamp and tungsten lamp to be lit simultaneously, enabling high-sensitivity wavelength-programming detection fro ultraviolet light and the entire visible-light range. Wavelength range 190 to 900nm.

ادامه مطلب  

1290 HPLC  

The Agilent 1290 Infinity LC system provides the highest levels of speed, resolution, flexibility and sensitivity for any LC and LC/MS application. 

 

Used Agilent 1290 Infinity Series HPLC System

hplcagilent1290.hplchplc.com/page-461081.html

 

agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1290 HPLC , Agilent 1290 LC System,Used Agilent 1290, Infinity Series, HPLC System This ...

----------------------

1290 Infinity Series HPLC

hplcpumps.hplchplc.com/list/1290+Infinity+Series+HPLC.html

 

 Used Agilent 1290 Infinity Series HPLC System · Download Hplc Agilent Technologies1290 Infinity II LC System Brochure and Guide. Downloa

ادامه مطلب  

Agilent HPLC 1260  

The Agilent 1260 Infinity HPLC-Chip/MS system is a re-useable microfluidic chip-based technology for high sensitivity nanospray LC/MS. The Agilent 1260 replaced the Agilent 1200 and delivers results faster to save time and money. 

Agilent Technologies 1260 Series

hplcagilent1260.hplchplc.com/

 

agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1260 HPLC , Agilent 1260 LCSystem,Used Agilent 1260, Infinity Series, HPLC System,DAD ...

________________________________

agilent 1260 hplc

watershplc.hplchplc.com/list/agilent+1260+hplc.html

 

This Agilent 1260 HPLC included: Binary pump with switch valve 1260 Binary Pump Thermostatted Column Compartment (TCC SL+)

ادامه مطلب  

Evaporative Light Scattering Detector (ELSD)  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds where UV detection might be a restriction and therefore compounds does not very efficient absorb UV radiation, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols. ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI).

ELSD | Evaporative Light Scattering Detector

elsd.hplchplc.com/

 

... Scattering Detector. An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC).

--------------------------

P

ادامه مطلب  

Shimadzu Photodiode Array SPD-M20A LC Detector  

General-purpose model
The SPD-M20A is a general-purpose model incorporating a deuterium lamp. The light-source compensation function achieves a noise level of 0.6×10-5AU. Cell temperature control ensures baseline stability.
Key Benefits:
Noise level of 0.6×10-5 AU
Superior Linearity - 2.0AU
Ethernet interface & web control functions
Can be incorporated into non-Shimadzu HPLC systems
Light source: Deuterium (D2) lamp, tungsten (W) lamp
Wavelength range: 190 to 800 nm
Wavelength accuracy: 1 nm max.
Wavelength precision: 0.1 nm max
Cell temperature control range: 5°C above room temperatre to 50°C
Operating temperature 4°C to 35°C
Power requirements: 100 VAC, 150 VA, 50/60 Hz
The SPD-M20A PDA is the third member of the UV-Vis absorbance detectors. The M20A, like

ادامه مطلب  

1100 HPLC  

Introduced in 1995, Agilent's 1100 series HPLC system is considered the world's most popular HPLC. They are incredibly robust machines that can last a long time. Finding a used Agilent 1100 on LabX is easy and maintenance for older machines consists of changing seals, filter solvents, lamps, and the odd part in the vacuum degasser. Parts from the Agilent 1050 are often shared with the Agilent 1100 which make it easy to maintain.

-------------

Agilent Technologies 1100 Series

hplcagilent1100.hplchplc.com/

Used Agilent 1100 Series HPLC with Multiple Wavelength Detector System. 1100 hplc,agilent hplc,hplc system,used hplc,used agilent hplc. HPLC System with ...

-------------

Used Agilent 1100 S

ادامه مطلب  

What Is Evaporative light scattering detector (ELSD)?  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds that do not absorb UV radiation and therefore cannot be detected by UV detectors, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols.ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI)./wikipedia

ادامه مطلب  

HPLC UV, VIS, and PDA Detectors  

The UV, VIS, and PDA detectors are categorized as absorbance detectors. They provide good sensitivity for light-absorbing compounds at ~pg level. They are easy to operate and provide good stability. UV detector is a very commonly used detector for HPLC analysis. During the analysis, sample goes through a clear color-less glass cell, called flow cell. When UV light is irradiated on the flow cell, sample absorbs a part of UV light. Thus, the intensity of UV light observed for the mobile phase (without sample) and the eluent containing sample will differ. By measuring this difference, the amount of sample can be determined. Since the UV absorbance also differs depend on what wavelength is used, it is important to choose an appropriate wavelength based on the type of analyte. A standard UV det

ادامه مطلب  

Shimadzu Refractive Index LC Detector 20A (RID-20A)  

Inheriting the stability and extensibility that are the strengths of the Prominence series, the new Shimadzu Refractive Index LC Detector 20A (RID-20A) model of differential refractive index detector is designed with a new reference-cell auto-purge feature and validation support function.

Specifications :

 
Pressure Relief Valve :
The RID-20A incorporates various safety features. Its maximum pressure is five times that of former Shimadzu products and, as a standard feature, it incorporates a sensor that detects leakage from the cell unit. For extra safety, a pressure relief valve that prevents problems related to back-pressure irregularities is also available as an option.

ادامه مطلب  

Shimadzu CDD-10AVP Conductivity LC Detector  

Handles a Wide Variety of Analysis Options
The CDD-10AVP conductivity detector achieves an even higher level of sensitivity and makes it possible to perform a wide variety of analysis scenarios with a single unit. An option card enables the simultaneous 2-channel measurement of anions and cations, and a suppressor option allows expansion to a suppressor system for ultra-high sensitivity work. Organic acids can be analyzed using Shimadzu's unique post-column pH-buffered electroconductivity method.
Low noise, low drift and wide dynamic range assure proven performance of the Shimadzu VP series HPLC. Data acquired by the CDD-10AVP is transferred to the SCL system controller via an optical fiber interface and are digitally processed by workstation. By adding the optional cell (dual kit NS

ادامه مطلب  

Shimadzu Nexera XR  

The Most Accessible UHPLC Available 
Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods.
Expanding the Possibilities of UHPLC :


Superb Functionality

Accuracy and reproducibility of solvent delivery and injection volume are particularly important performance aspects. Shim

ادامه مطلب  

Shimadzu Prominence nano  

The Most Accessible UHPLC Available 
Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods.
Expanding the Possibilities of UHPLC :


Superb Functionality

Accuracy and reproducibility of solvent delivery and injection volume are particularly important performance aspects. Shim

ادامه مطلب  

Shimadzu Nexera X2  

Maximizing the Potential of UHPLC/HPLC Analyses Ultra High Performance Liquid Chromatograph Nexera X2

Intelligence

Intelligent data solutions, including a new  i-PDeA peak calculation method for enhanced separation power, accelerate workflows.

Sensitivity

The ultimate platform for high-sensitivity UHPLC and HPLC detection.

Resolution

Enhanced peak resolution achieved by minimizing external column dispersion.

Stability

Superior stability for UHPLC/HPLC analyses.
Shimadzu Nexera X2 Utilizing patented technologies, and driven by customer input, Shimadzu has developed the Nexera X2 series to deliver superior, real-world performance across a wider application range, while delivering unparalleled flexibility and reliability in UHPLC/HPLC analyses. With no limi

ادامه مطلب  

Shimadzu Photodiode Array SPD-M30A LC Detector  

Supports diverse applications from HPLC to UHPLC
The SPD-M30A detector achieves a 0.4×10-5 AU noise level. The SR-Cell (Sensitivity and Resolution Cell) significantly cuts peak dispersion. This model supports analysis from conventional LC to ultra-fast and UHPLC analysis. The optional high-sensitivity cell has an 85 mm optical path length and is able to detect trace components that were conventionally difficult to detect. The TC-Optics function further improves baseline stability.

ادامه مطلب  

Two Types Of UV Detectors Are Commonly Used Today  

Two types of UV detectors are commonly used today:

Variable-wavelength (sometimes called "spectrophotometric" detectors )
Photodiode Array (sometimes simply called "diode array" detectors.)
Variable-wavelength detectors are less expensive; they are the standard detector type for quantitative analysis and routine assays. Photodiode array detectors are more versatile, because they allow simultaneous acquisition of both chromatographic and spectral information; they are frequently used in method development.
The detector wavelength is an important characteristic of an HPLC separation. As a general rule, the wavelength is set to the absorbance maximum of the analyte. Using the wrong wavelength may result in decreased peak sizes, or even no peaks at all!
Because different compounds can h

ادامه مطلب  

Shimadzu Evaporative Light Scattering LC Detector LTII (ELSD-LTII)  

In the history of high-performance liquid chromatographs, which dates to the early 1960s, refractive index detectors (RI detectors) have often been used as general-purpose detectors. RI detectors enable the detection of components that do not possess UV absorbance and give a proportional relationship between the heights of detected peaks and the quantities of detected components. So, in comparison with absorbance detectors (UV detectors), they offer advantages such as the ability to ascertain unknown component quantities and obtain molecular weight distributions for macromolecules. On the other hand, they also have various disadvantages. For example, they cannot be used for gradient analysis, the baselines they produce are susceptible to the influence of fluctuations in the ambie

ادامه مطلب  

Shimadzu RF-20A/RF-20Axs Fluorescence LC Detector  

The RF-20A/20AXS fluorescence detectors offer world-leading sensitivity and ease-of-maintenance. The RF-20AXS is a high-sensitivity model that incorporates a temperature-controlled cell with a cooling function as standard.

RF-20A / Standard model:
The RF-20A, which offers best-in-class sensitivity, features a water Raman S/N ratio of at least 1200, as well as excellent ease-of-use with such features as maintenance from the front panel and adoption of a long-life lamp.

RF-20AXS / Achieves World-Leading Sensitivity :
Offering world-class levels of sensitivity and easy maintenance, the RF-20AXS features a water Raman S/N ratio of at least 2000 and a temperature-controlled cell with a cooling function. This maintains a constant detector cell temperature, even if the room temperature

ادامه مطلب  

HPLC Fluorescence Detector (FLD)  

The advantage of fluorescence method is its high sensitivity for selective groups of compounds at ~fg level. By using a specific wavelength, analyte atoms are excited and then emit light signal (fluorescence). The intensity of this emitted light is monitored to quantify the analyte concentration. Most pharmaceuticals, natural products, clinical samples, and petroleum products have fluorescent absorbance. For some compounds which do not have fluorescence absorbance or low absorbance, they can be treated with fluorescence derivatives such as dansylchloride. The system is easy to operate and relatively stable.
Principles Of HPLC  Fluorescence Detector (FLD)
Fluorescence detectors are probably the most sensitive among the existing modern HPLC detectors. It is possible to detect even a presenc

ادامه مطلب  

Agilent HPLC  

Find great deals on HPLCHPLC.COM for Used and Refurbished  Agilent HPLC in Analytical Lab Instruments. Shop with confidence. 





 ________________________________________
agilent 1200 hplc


hplc.hplchplc.com/list/agilent+1200+hplc.html
 
This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings: G1379B Degasser with Tray G2226A ...
________________________________________



Agilent Technologies 1260 Series


hplcagilent1260.hplchplc.com/
 
agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1260 HPLC , Agilent 1260 LCSystem,Used Agilent 1260, Infinity Series, HPLC System,DAD ...
________________________________________



USED AGILENT HP 1200 HPLC Series SYSTEM


hplccolumn.hplchplc.com/page-461164.html
 
used

ادامه مطلب  

HPLC (high performance liquid chromatograph ) Instrument Operation  

General components of a high performance liquid chromatograph.
HPLC solvent delivery systems.
How automatic injectors work.
Common HPLC detectors.
Downlod powerpoint file about hplc instrumentation .
source:Philadelphia University

ادامه مطلب  

Agilent HPLC 1200  

The Agilent 1200 Series HPLC System was introduced in 2010 with a modular design allowing users to define a configuration ideally suited to meet their HPLC and UHPLC applications and requirements. 

agilent 1200 hplc

hplc.hplchplc.com/list/agilent+1200+hplc.html

This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings: G1379B Degasser with Tray G2226A ...

_____________________________________

USED AGILENT HP 1200 HPLC Series SYSTEM

hplccolumn.hplchplc.com/page-461164.html

used hplc,agilent 1200, 1200 hplc,used agilent 1200 hplc,on sale hplc. We offer an Agilent Technologies 1200 Series HPLC System in excellent working ...

____________________________

ادامه مطلب  

waters hplc  

Whatever your application, Alliance® HPLC, BreezeTM 2 HPLC, and AutoPurificationTM systems offer you proven solutions for all of your HPLC requirements - for today and the future. These systems are synonymous with dependable, routine performance and versatility.

Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance HPLC family meets the rigorous requirements of routine chromatography and the performance standards of new-product research and method development.

Breeze 2 HPLC Comes with a pump, detector, injector, and is pre-configured for different levels of HPLC. It includes Breeze Software, an intuitive interface that is easy to set-up, learn, and use.
waters


waters.hplchplc.com/list/waters.html
 
Waters Alliance HPLC THAT WORKS AS HARD AS YOU

ادامه مطلب  

High Performance Liquid Chromatography Detectors ( HPLC Detectors)  

1. HPLC UV, VIS, and PDA Detectors
2. HPLC Refractive-Index Detector
3. HPLC Evaporative Light Scattering Detector
4. HPLC Multi-Angle Light Scattering Detector
5. HPLC Mass Spectrometer
6. HPLC Conductivity Detector
7. HPLC Fluorescence Detector
8. HPLC Chemiluminescence Detector
9. HPLC Optical Rotation Detector
10.HPLC Electro Chemical Detector
The actual separation of each component in the sample is carried inside a column; however this separation needs to be "collected" for us to be able to see it. The detectors are used for this purpose. The separated coponents are monitored and expressed electronically. There is no universal detector that can monitor all compounds and there are many detectors used for LC analysis. Some are listed below.


Type
Common Abbreviation

Ultra Violet
UV

V

ادامه مطلب  

Chromatography terms / analyte / Analytical chromatography / bonded phase / chromatogram /...  

The analyte is the substance to be separated during chromatography. It is also normally what is needed from the mixture.
Analytical chromatography is used to determine the existence and possibly also the concentration of analyte(s) in asample.
A bonded phase is a stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing.
A chromatogram is the visual output of the chromatograph. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture.
 Plotted on the x-axis is the retention time and plotted on the y-axis a signal (for example obtained by a spectrophotometer, mass spectrometer or a variety of other detectors) corresponding to the response creat

ادامه مطلب  

principle of hplc:High Performance Liquid Chromatography (HPLC)  

High performance liquid chromatography (HPLC) has become a very versatile and powerful separation and analytical method over the years. It is an advanced form of liquid chromatography (LC).
Instead of introducing the solvent into the column and allowing it to drip down under the influence of gravity, in HPLC the sample is forced through the column under high pressures of nearly 400 atm, resulting in faster and more efficient separation.
This technique is also called high pressure liquid chromatography.

The Principle of HPLC
HPLC follows the same basic principle as chromatography. Different components in the sample have varying affinities to the adsorbent material. This causes a difference in the flow rate for each component which leads to their separation as they come out of the column.

ادامه مطلب  

What Is Column chromatography?  

For more details on this topic, see Column chromatography.
Column chromatography is a separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open tubular column). Differences in rates of movement through the medium are calculated to different retention times of the sample.
In 1978, W. Clark Still introduced a modified version of column chromatography called flash column chromatography (flash).The technique is very similar to the traditional column chromatography, except for that the

ادامه مطلب  

HPLC Column | Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column  

ZORBAX Carbohydrate Analysis Columns are reproducible, efficient, and flexible. These columns use ZORBAX porous silica microsphere technology. Silica manufacture, bonding and packing are all performed in our ISO9001 facilities. ZORBAX Carbohydrate Analysis Columns can handle high volume injections as much as 50 µL on a 4.6 x 150 mm column.
Features Of Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column:
Reproducible
Efficient – uses ZORBAX porous silica microsphere technology; silica manufacturing, bonding and packing are all performed in Agilent's ISO 9001 facilities
Flexible – can handle high volume injections – as much as 50 μL on a 4.6 x 150 mm column
Recommended for use with refractive index detectors (RID)

ادامه مطلب  

HPLC Column | Agilent MicroBore Small Molecule Separations LC Column  

MicroBore (1 mm id) columns are a good choice when sample sizes are limited. They will improve detection limits 5 times over 2.1 mm id columns when the same sample mass is used. This increase in sensitivity can be critical. MicroBore columns use low flow rates (typically ~50 µL/min). Therefore, these columns are ideal for use with detectors requiring low flow ratessuch as some mass spectrometers. MicroBore columns perform optimally with HPLC systems purchased or modified for microbore use. Guard columns are now available with an adjustable tube stop depth to provide a perfect zero dead volume connection every time.
Features Of Agilent MicroBore Small Molecule Separations LC Column:
High sensitivity for small sample sizes
Compatible with LC/MS interfaces
Wide variety of bonded phases
G

ادامه مطلب  

principle of hplc:How Does High Performance Liquid Chromatography Work?/what is Detector,Chromatogram?  

How Does High Performance Liquid Chromatography Work?

The components of a basic high-performance liquid chromatography [HPLC] system are shown in the simple diagram in Figure E.
A reservoir holds the solvent [called the mobile phase, because it moves]. A high-pressure pump [solvent delivery system or solvent manager] is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute. An injector [sample manager or autosampler] is able to introduce [inject] the sample into the continuously flowing mobile phase stream that carries the sample into the HPLC column. The column contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.

ادامه مطلب  

HPLC Optical Rotation Detector (ORD)  

Specific for the optical isomer measurement. The column can separate R- and L- type optical isomers, but the general detectors (e.g., UV) cannot distinguish which is R nor L. OR detector provides this information.
In chiral chromatography, the technology of detecting chiral molecules using optical devices has lagged behind until now. The stability and sensitivity of this type of instrument is an important issue in chiral chromatography. However, with the availability of the single wavelength LED, increased sensitivity and increased economics with simplified instrument design was possible. In addition, a newly designed glass coated steel flow cell has produced a superior, third generation optical rotation detector. Aided with the Faraday compensation technique, this instrument gives high o

ادامه مطلب  

Waters Breeze 2 HPLC  

The Breeze™ 2 HPLC System delivers technology and performance in an affordable, compact, and user-friendly system platform. Complete with software, pump, detector and injector, the system comes pre-configured for different levels of HPLC operational needs.
From teaching purposes to daily analytical work, the Breeze 2 HPLC System integrates simplicity, sensitivity, accuracy, and reliability. It is ideal for any organization seeking a quality HPLC platform with limited budget and chromatography experience including university laboratories, government laboratories, or start-up companies.
The Waters® Breeze 2 HPLC System delivers routine analyses and robust performance day after day, providing the  onfidence you need to get the job done. Used in laboratories worldwide, chromatograp

ادامه مطلب  

Principle of HPLC:High Performance Liquid Chromatography (HPLC) : Principle, Types, Instrumentation and Applications  

Chromatography is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of variuos components. Chromatographic separations can be carried out using a variety of stationary phases, including immobilized silica on glass plates (thin-layer chromatography), volatile gases (gas chromatography

ادامه مطلب  

Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes  

Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
We occasionally get calls or emails from folks that are seeing lower than expected response for peaks of interest. This may be a matter of method development if you have not done this analysis previously. Suggestions in the post are provided assuming you are working with an established method and have had successful results over an extended period of time (until recently). These suggestions also assume that the response is low for quantitation standards as well as in prepared QC samples. If

ادامه مطلب  

Troubleshooting HPLC- Loss in Response for all Analytes  

Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
We occasionally get calls or emails from folks that are seeing lower than expected response for peaks of interest. This may be a matter of method development if you have not done this analysis previously. Suggestions in the post are provided assuming you are working with an established method and have had successful results over an extended period of time (until recently). These suggestions also assume that the response is low for quantitation standards as well as in prepared QC samples. If t

ادامه مطلب  

Reversed Phase Chromatography (RPC)  

Reversed phase HPLC (RP-HPLC) has a non-polar stationary phase and an aqueous, moderately polar mobile phase. One common stationary phase is a silica which has been surface-modified with RMe2SiCl, where R is a straight chain alkyl group such as C18H37 or C8H17. With such stationary phases, retention time is longer for molecules which are less polar, while polar molecules elute more readily (early in the analysis). An investigator can increase retention times by adding more water to the mobile phase; thereby making the affinity of the hydrophobic analyte for the hydrophobic stationary phase stronger relative to the now more hydrophilic mobile phase. Similarly, an investigator can decrease retention time by adding more organic solvent to the eluent. RP-HPLC is so commonly used that it is oft

ادامه مطلب  
صفحات ادامه نتايج:  1